Clove oil is a colorless to light yellow oily liquid, with clove aroma and special spicy aroma. Placed in the air for a long time can be oxidized and dark in color, easy to dissolve in ethyl ether, acetone, ethyl acetate and other organic solvents, soluble in ethanol, difficult to dissolve in water.In medicine, it is used for antisepsis and oral disinfection. In industry, it is mainly used for preparing toothpaste and soap flavor or as raw material for synthesis of vanil

Clove oil is the essential oil extracted from the buds of cloves. It can treat toothache, bronchitis, neuralgia, stomach acid, resist respiratory system and urinary system infection, relieve discomfort and pain caused by dysentery, improve weak constitution and anemia, and aphrodisiac (sexual Impotence, cold feeling), insect repellent. Promote blood circulation, treat skin ulcers and wound inflammation, treat scabies, and improve rough skin.

Clove oil contains a variety of active ingredients, which have insecticidal, bacteriostatic, and antioxidant effects, and there are no residues and drug resistance problems. As well as human health and safety issues, Caenorhabditis elegans was used as a model organism to study the poisonous effect of clove oil on nematodes. The experimental results showed that the semi-lethal concentrations (IC50) of clove oil and eugenol to Caenorhabditis elegans were 55.74 and 29.22 mg/L respectively; they could inhibit catalase, glutathione and superoxide Dismutase activity disrupts the dynamic balance of oxidation and anti-oxidation and reduces nematode oviposition. Through RNA-seq sequence analysis, the effects of clove oil and eugenol on nematodes were interpreted from the genetic level, and it was found that the genes that act on the metabolic function of nematodes were regulated, such as E01G6.1, cht-1, C40H1.8, lipl-5, Fat-2, txt-8, fat-4, acox-1.2, dagl-2, pigw-1, etc.; the regulation of genes such as hsp-70 and F44E5.5 makes the production of proteins in nematodes, especially heat shock proteins and jobs are affected. Clove oil is highly toxic to Caenorhabditis elegans, and can enter the body of the nematode to destroy it, reducing the harm of the nematode from the root. It can be seen that clove oil can be used in combination with traditional chemical agents, thereby reducing the amount of traditional chemical agents, and has great development value as a green insecticide in the application of agricultural fields.